Abstract: Based on thermal simulation experiment of closed gold tube system under the given pressure(50 MPa)and analysis of methane carbon isotopes with GC-IRMS isotope mass spectrography，combined with the kinetic modeling calculation by using the GOR-Isotope Kinetics software，this paper obtained the carbon isotopic kinetic parameters of methane generated from Triassic-Jurassic coal measures source rocks in the Kuqa depression，Tarim Basin．The average activation energies of Jurassic coal，Jurassic mudstone and Triassic mudstone samples selected from Well Yinan 2 were 227.90 kJ/mol，204.84 kJ/mol，230.63 kJ/mol，respectively．The kinetic modelling results of methane carbon isotopes were generally accorded with the laboratory pyrolysis data．It is demonstrated that the laboratory pyrolysis data could be extrapolated and applied to the actual geological condition by using the method of carbon isotope kinetics．Combined with the geological background，the source，maturity，and accumulation time of natural gas in Kela-2 gas field were further discussed．Natural gas of Kela-2 gas field was mainly derived from the Middle and Lower Jurassic coal-bearing source rocks，and belonged to the staged cumulative gas．The gas was chiefly accumulated in the last 5Ma，with its Ro value ranging from 1.3％ to 2.5％ ．On the basis of the above research．a kinetic model of natural gas migration and accumu1ation in Kela-2 gas field was established ．This kind of study may provide a new idea for natural gas quantitative assessmentand dynamic research．