Abstract: To better explore the effect of culture method (static and shake cultures) on species and morphologies of carbonate minerals
induced by bacteria, a batch of oscillatory experiments were carried out for a duration of 40 days in B4 medium under the mediation of
three kinds of bacteria-Bacillus cereus (strain GW-1), Lysinibacillus sp. ( strain GW-2) and Microbacterium sp. (strain GW-3). At the
same time, aseptic experiment without the inoculation was done as the control. The mineral species of carbonate were determined by
X-ray diffraction (XRD), and morphologies of precipitated carbonates were observed using scanning electron microscopy (SEM). The
results obtained under oscillatory incubation condition were compared with those obtained under static condition. The main results
were as follows: (1) All three bacterial strains could induce precipitate of calcium carbonate, but they had different abilities. Their
mineralization abilities were in the order of GW-2＞GW-1＞GW-3; (2) At the two culture methods, strains GW-1 and GW-2
induced the formation of calcite, while strain GW-3 mediated the formation of vaterite. The results suggested that bacterial specieswere the important factors affecting mineral species. Culture methods almost had no effect on mineral species; (3) The morphologies
of minerals induced by strains GW-1 and GW-2 were diversified with shake culture. However, single morphology existed in the
samples with static culture. The morphology of all minerals formed by strain GW-3 was spherical shape and was not related to the
culture methods used.

Key words: calcite, vaterite, morphology, bacteria, shake culture, static culture