Acidithiobacillus ferrooxidans ATCC23270 与矿物吸附过程的热量变化及不同条件下代谢热的基础研究

Acidithiobacillus ferrooxidans ATCC23270 与矿物吸附过程的热量变化及不同条件下代谢热的基础研究

王秀美1,3，柳建设2*，李邦梅1，朱建裕1，王铧泰1，闫　颖1，邱冠周1

1. 中南大学资源加工与生物工程学院，长沙 410083； 2. 东华大学环境科学与工程学院，上海 201620；3. 长春黄金研究院，长春 130012

收稿日期:2009-06-20 修回日期:2009-06-20 出版日期:2009-06-20 发布日期:2009-06-20

Study on the enthalpy variation during adsorption processes of Acidithiobacillus ferrooxidans ATCC23270 on the surface of minerals and its metabolic thermogenesis under different conditions

WANG Xiu-mei1, 3, LIU Jian-she2*, LI Bang-mei1, ZHU Jian-yu1,WANG Hua-tai1, YAN Yin1, QIU Guan-zhou1

1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; 2. School of Environmental Science and Engineering, Donghua University, Shanghai 201620, China; 3. Changchun Gold Research Institute, Changchun 130012, China

Received:2009-06-20 Revised:2009-06-20 Online:2009-06-20 Published:2009-06-20

摘要/Abstract

摘要： 用微量热技术研究了不同条件下Acidithiobacillus ferrooxidans ATCC23270（以下简写为A. ferrooxidans ATCC23270）与硫化矿吸附过程的热量变化以及不同细菌浓度、不同的初始pH 值及不同的培养条件下细菌的代谢产热情况。研究发现，矿浆浓度0.03 g/mL，细菌浓度1.7×108 个/mL 的时候细菌与矿物的吸附放热最大。不同条件培养的细菌胞外多聚物的组成不同，与矿物吸附的反应热也不相同，黄铁矿培养细菌胞外多聚物含量最高，反应热也最高，说明细菌胞外多聚物在吸附过程中起重要作用。用微量热法能够很好地反映出细菌生长代谢过程中每个微观时刻的热量变化。对于A. ferrooxidans ATCC23270，最佳的代谢产热条件为：pH 值为2.0 和2.3，细菌浓度为3.0×108 个/mL。不同培养条件下的细菌的生长代谢热不同，2039+FeSO4 培养的代谢热最大、放热最快。

Abstract: The influencing factors of the enthalpy variation during adsorption processes of Acidithiobacillus ferrooxidans ATCC23270 on the surface of sulfide minerals and the metabolic thermogenesis of the bacteria under different conditions (different concentrations of the bacteria, different initial pH values and different media) were studied using microcalorimetry. The results show that the concentration of pulp at 0.03 g/mL and concentration of bacteria at 1.7×108 were the best environment for enthalpy variation during adsorption processes of A. ferrooxidans ATCC23270 on the surface of minerals. A. ferrooxidans cultured in different media with different contents of extracellular polymers (EPS) create different heat of reaction attaching on the surface of minerals. Bacteria cultured with pyrite having the highest EPS create the biggest heat of reaction, which proves the important role of EPS in the process of adsorption. The growth thermograms of bacteria can be determined by using the mircocalorimetric method. These perfect thermogram curves reflect the changes of bacteriaI growth patterns. For A. ferrooxidans ATCC23270, the best condition for the metabolic thermogenesis is at pH = 2.0 or 2.3, and concentration of bacteria at 3.0 × 108 cell/ mL. The bacteria cultured in different media create different growth thermograms. Media of 2039+ FeSO4 is the best one for metabolic thermogenesis.

王秀美,柳建设，李邦梅，朱建裕，王铧泰，闫　颖，邱冠周. Acidithiobacillus ferrooxidans ATCC23270 与矿物吸附过程的热量变化及不同条件下代谢热的基础研究[J]. J4.

WANG Xiu-mei1, 3, LIU Jian-she2*, LI Bang-mei1, ZHU Jian-yu1,WANG Hua-tai1, YAN Yin1, QIU Guan-zhou1. Study on the enthalpy variation during adsorption processes of Acidithiobacillus ferrooxidans ATCC23270 on the surface of minerals and its metabolic thermogenesis under different conditions[J]. J4.