Abstract: Phosphatidylcholine (PC) is one of the basic components of the biomembrane, which plays important roles in the biomineralization. As a double-hydrophilic biomacromolecule, PC can form liposomes in solution and lipid membrane layer at the interface of solution/air. In this paper, using X-ray diffraction (XRD), scanning electron microscope (SEM) and dynamic light scattering (DLS), we systematically investigated mineralization of calcium carbonate in the presence of PC and/or glycylglycine by a biomimetic mineralization approach. As a result, we found that phosphatidylcholine and glycylglycine obviously have effects on surface microstructure of calcium carbonate,and that the electrostatic force interaction between the PC molecules at the interface of solution/air and the dissolved glycylglycine synergically inhibits mineralization of calcium carbonate minerals.