Abstract: Subacute toxicity of combined effect of strontium (Sr), boron(B) and fluorine(F) was tested on mice by drinking water containing Sr2+, B3+ and F-. The results showed that F content in bones and activity of alkaline phoshatase (Al, P)in kidney increased significantly(p＜0.01)after the test of water containing 200 ppm F- for six months. ALP activity in serum also Increased sharply (P< 0.01)after 4.5 months, Sr and B added to the water with final concentration of 80 ppm and 100ppm respectively could induce the decrease of F content in bones and activity of Al P in kidney and serum. This indicates that Sr and B are both antidotes to F.