Abstract: Geo-temperature is a decisive factor for the formation of oi1 and gas. Distribution of geotemperature is a geophysical field changing with time and boundary conditions. Present geothermal field is the result of the origin and evolution of sedimentary basin, and a basic constraint for the paleo-geothermal field modeling and inversion. The thermal resistance method and bottom hole temperatures are used to determine heat flow and subsurface temperature. The dispersive model of sedimentary rock thermal conductivity is applied to determine the in situ thermal conductivity of Tertiary strata in Dongying Depression, which ranges from 1.6w/mK to 2.4W/mK for Minghuazhen Formation (Nm), from 1.6W/m K to 3.3mW/mK for Guantao Formation(Ng) and from 2.5W/mK to 3.1mW/mK for Dongying Formation (Ed). It has been found that in situ thermal conductivity in Dongying Depression is mainly related to sandy content and depth of the Tertiary sedimentary strata. Based on the results of above calculation and the measured temperature data, temperatures on different interface, and some particular depths are calculated at the 170 temperature measured wells using thermal resistance method. The temperature changes from 2212 to 5512 on the top surface of Minghuazhen Formation (Nm), and from 41℃ to 72℃ at its bottom surface. Temperatures on the bottom surface of Guantao Formation(Ng) are from 53℃ to 86℃. On the bottom surface of Dongying Formation(Ed), geo-temperatures are from 50 to 104℃. In Dongying Depression, geo-temperature ranges from 4912 to 8012 at the depth of lO00m, from 72℃ to 106℃ at the depth of 2000m , from 89℃ to 146℃ at the depth of 3000m, from 103℃ to 186℃ at the depth of 4000m. from 119℃ to 222℃ at the depth of 5000m The geo-temperature distribution at the same depth is closely related to the heat flow.