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J4 ›› 2011, Vol. 17 ›› Issue (1): 101-106.

• 微生物—矿物相互作用专栏 • 上一篇    下一篇

红壤中微生物群落对半导体矿物日光催化作用的响应

 曾翠平, 鲁安怀, 李  艳, 吴  婧, 王  鑫, 丁竑瑞, 颜云花   

  1. 造山带与地壳演化教育部重点实验室,北京大学  地球与空间科学学院
  • 收稿日期:2011-01-07 修回日期:2011-02-23 出版日期:2011-03-20 发布日期:2011-03-20
  • 通讯作者: 鲁安怀,教授,博士生导师,主要从事环境矿物学教学和研究;
  • 作者简介:曾翠平,1985年生,女,博士研究生,从事微生物与矿物交互作用研究;
  • 基金资助:

      国家重点基础研究发展计划资助项目(973项目)(2007CB825602)

Response of microbial community to sunlight catalysis of semiconductor minerals in red soil

ZENG Cui-ping, LU An-huai, LI Yan, WU Jing, WANG Xin, DING Hong-rui, YAN Yun-hua    

  1. Key Laboratory of Orogenic Belts and Crustal Evolution of MOE, School of Earth and Space Sciences, Peking University
  • Received:2011-01-07 Revised:2011-02-23 Online:2011-03-20 Published:2011-03-20
  • Contact: Lu Anhuai, Professor.

摘要:

本研究选取天然红壤作为研究对象,分析其中的含铁半导体矿物光催化作用对本源微生物群落结构的影响。采用聚
合酶链式反应/变性梯度凝胶电泳(PCR-DGGE),研究了不同原始光照条件的红壤中微生物群落在外源电子作用下群落结
构的改变。DGGE图谱的主成分分析表明,两个不同原始光照环境的土壤样品的微生物群落结构由于原始环境的差异而不同:
原始环境为强光照的样品中微生物群落结构受外源电子影响较小;原始环境为弱光照的样品中微生物群落结构受外源电子
影响较大。这一群落结构改变的差异可能由于原始环境为强光照时半导体矿物光催化作用产生光生电子传递到环境中持续
影响周围的微生物群落,而原始弱光照环境中则缺少光生电子的作用。

关键词: 红壤, 微生物群落, PCR-DGGE, 外源电子, 半导体光催化

Abstract:

The natural  red soil was used  in  this study as a  research object, and  the  influence of semiconductor photocatalysis
of  iron-bearing minerals on microbial community was analyzed. Using  the polymerase chain  reaction/degeneration gradient gel
electrophoresis  (PCR-DGGE),  the microbial community  from  the original  red soil samples under different  light conditions was
investigated after being disposed with exogenous electrons supplement. Principal component analysis  (PCA) of DGGE banding
patterns indicated that the difference of the microbial community structure in two soil samples was due to different original light
environments.  In  the  red soil with strong  light, no significant change of  the microorganism community structure was observed
by processing with exogenous electrons; while in the original environment with weak light, the structure of microbial community
was significantly affected by  the exogenous electrons. The different response of microbial community structure  to  the exogenous
electrons may be attributed to the photoinduced electrons generated by the semiconducting mineral photocatalysis in the primitive
strong  irradiation environment, which continued  to affect  the surrounding microbial communities; while photoinduced electrons
were inadequate in the weak irradiation environment.

Key words: red soil, microbial community, PCR-DGGE, exogenous electrons, semiconductor photocatalysis

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