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J4 ›› 2011, Vol. 17 ›› Issue (1): 107-111.

• 微生物—矿物相互作用专栏 • 上一篇    下一篇

含钾矿粉对胶质芽孢杆菌分泌胞外多糖的影响

 胡  星, 连  宾, 郁建平, 胡  婕   

  1. 贵州大学 生命科学院
  • 收稿日期:2010-11-25 修回日期:2011-01-20 出版日期:2011-03-20 发布日期:2011-03-20
  • 通讯作者: 连宾,研究员,
  • 作者简介:胡星,1982年生,女,硕士研究生,应用生物化学与生化分离工程专业;E-mail: huxing1624@126.com
  • 基金资助:

      国家高技术研究发展项目863课题(2008AA06Z108);国家自然科学基金委创优群体项目(40721002)和中国科学院“百人计
    划” 项目联合资助

Effect of potasium-bearing mineral powder on the exo-polysaccharides secreted by Bacillus mucilaginosus

HU Xing, LIAN Bin, YU Jian-ping, HU Jie   

  1. College of Life Science, Guizhou University
  • Received:2010-11-25 Revised:2011-01-20 Online:2011-03-20 Published:2011-03-20
  • Contact: Senior Research Fellow

摘要:

研究了不同实验条件下,含钾矿粉对胶质芽孢杆菌(Bacillus mucilaginosus)分泌胞外多糖的影响,以进一步揭示细
菌与矿物的相互作用机理。实验分别采用无矿(不加含钾矿粉)无钾(不含可溶性钾)、无矿有钾(有可溶性钾)、有矿(100
mL培养液加0.3 g含钾矿粉)无钾及有矿有钾4组培养基培养胶质芽孢杆菌,进行产胞外多糖的分析比较。研究发现,在有
钾长石粉但不含钾离子的培养条件下,胶质芽孢杆菌产生的多糖和蛋白质含量最高;在既有钾矿粉又有可溶性钾离子存在
的培养条件下,菌体产生的蛋白质和胞外多糖含量稍低;在无钾矿粉的条件下,无论有没有钾离子的两种培养基中,蛋白
质和多糖含量都很低。研究结果表明,有无钾矿粉的不同培养条件能够引起胶质芽孢杆菌分泌胞外多糖的单糖组成成分产
生明显差异,胶质芽孢杆菌胞外分泌物与细菌对含钾矿粉中钾的利用密切相关。

关键词: 胶质芽孢杆菌, 胞外多糖, 解钾作用, 单糖组分

Abstract:

Under different conditions, effects of mineral powder containing potassium on  the exo-polysaccharides  (EPS) secreted
by Bacillus mucilaginosus were studied  to  further explain  the reciprocity between bacteria and potassium-baring minerals. In  the
research, B. mucilaginosus was cultured  in  four kinds of media  to analyze and compare  their EPS production. Results showed  that
bacteria secreted the highest output of both proteins and EPS in the culture with potassium-bearing minerals, but without potassium
ion. While  in  the medium with potassium  ion,  the yielded EPS were  lower. However,  in  the medium without potassium beaing
mineral powder and with or without potassium  ion,  the production of both proteins and EPS was proved  to be  the  lowest. List of
monosaccharide components of EPS secreted by B. mucilaginosus varied obviously in relation with different culture conditions. The
results demonstrated that extracellular secretion of the bacteria is closely related to the mechanism of potassium releasing.

Key words:  Bacillus mucilaginosus, exo-polysaccharides, potassium releasing mechanism, monosaccharide component

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