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J4 ›› 2011, Vol. 17 ›› Issue (1): 53-58.

• 微生物—矿物相互作用专栏 • 上一篇    下一篇

铀与酵母菌细胞表面相互作用研究

刘明学, 张  东, 康厚军, 张  伟, 李  烨, 庞小峰, 董发勤   

  1. 西南科技大学 生命科学与工程学院,四川
  • 收稿日期:2011-01-07 修回日期:2010-02-21 出版日期:2011-03-20 发布日期:2011-03-20
  • 通讯作者: 刘明学,1975年生,男,西南科技大学副教授,主要研究方向:矿物、核素-微生物相互作用;
  • 作者简介:刘明学,1975年生,男,西南科技大学副教授,主要研究方向:矿物、核素-微生物相互作用;E-mail: dragonlmx@126.com
  • 基金资助:

    国家自然科学基金委员会—中国工程物理研究院联合基金资助项目《微生物对放射性核素的吸附富集研究》(10776027)

The interaction between uranium and yeast cell surface

LIU Ming-xue,ZHANG Dong,KANG Hou-jun,ZHANG Wei,LI Ye,PANG Xiao-feng,DONG Fa-qin   

  1. Life Science and Engineering College, Southwest University of Science and Technology, Mianyang
  • Received:2011-01-07 Revised:2010-02-21 Online:2011-03-20 Published:2011-03-20
  • Contact: Liu Mingxue, Associate Professor.
  • About author:Liu Mingxue, Associate Professor.

摘要:

应用扫描电子显微镜(SEM),傅立叶红外光谱 (FTIR)和电子能谱(EDS)方法研究了酿酒酵母菌与铀酰离子的相
互作用。电镜结果表明,吸附铀后酵母菌细胞发生一定变化,随时间延长细胞异常加剧;细胞表面有大量铀结晶,结晶
量随铀浓度增加和作用时间延长而加大。能谱分析表明,酵母菌细胞表面结晶为含铀化合物。对照吸附前后的红外光谱
图,细胞表面的O—H键作用后向低波数移动29 cm-1,υs (—PO2-)、糖类的C—OH的伸缩振动均移动了约21 cm-1
,羧基(—COOH)的υC=O吸收带几乎完全消失;根据铀酰离子U—O键特征吸收峰推断形成的结晶可能为钙铀云母。

结果表明,UO22+离子与酵母菌细胞表面发生了显著的吸附作用。

关键词: 酿酒酵母菌, 铀, 吸附, 表面作用

Abstract:

The yeast (Saccaromyces cerevisiae) absorption of uranium was studied by using Scanning Electron Microscope (SEM),
Fourier Transform  Infrared Spectrometry  (FTIR) and Energy Dispersive X-ray Spectroscopy  (EDXS). The SEM  results showed 
that yeast cell surface was covered with uranium crystal and the quantity of crystal increased with concentration of uranium and
the interaction time. The surface morphology of yeast changed greatly with incubation time. EDXS analysis showed that the crystal
of uranium was formed via adsorption on the surface of S. cerevisiae. FTIR results revealed that the peak attributed to υs(O-H) of
yeast cell surface shifted about 29 cm-1 to lower wavenumbers. Both peaks of υ s(C-OH) and υs(-PO2-) shifted about 21 cm-1
 to lower wavenumbers, and the band attributed  toυ C=O of the carboxyl group almost disappeared. The formed crystal  is considered
to be autunite according to the FTIR characteristic absorption band. These results indicated that the UO22+ can be stabilized via
adsorption by S. cerevisiae

Key words: Yeast (Saccaromyces cerevisiae), uranium, absorption, surface-interaction

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